Human Mucus Protease Inhibitor in Airway Fluids Is a Potential Defensive Compound against Infection with Influenza A and Sendai Viruses
Identifieur interne : 001B49 ( Main/Exploration ); précédent : 001B48; suivant : 001B50Human Mucus Protease Inhibitor in Airway Fluids Is a Potential Defensive Compound against Infection with Influenza A and Sendai Viruses
Auteurs : Yoshihito Beppu ; Yasuhiro Imamura ; Masato Tashiro ; Takae Towatari ; Hiroyoshi Ariga ; Hiroshi KidoSource :
- The Journal of Biochemistry [ 0021-924X ] ; 1997.
Abstract
Tryptase Clara, a trypsin-like protease localized exclusively in and secreted from Clara cells of the bronchial epithelium of rat, proteolytically activates the infectivity of influenza A virus [H. Kido, Y. Yokogoshi, K. Sakai, M. Tashiro, Y. Kishino, A. Fukutomi, and N. Katunuma (1992) J. Biol. Chem. 267, 13573–13579]. We report here that human mucus protease inhibitor (MPI), a major inhibitor of granulocyte elastase in the lining fluids of the human respiratory tract, significantly inhibited proteolytic activation of the infectivity of influenza A and Sendai viruses by tryptase Clara in vitro and multi-cycles of mouse-adapted influenza A virus replication in rat lungs in vivo. Recombinant MPI and the C- but not the N-terminal domain of MPI inhibited both the proteolytic activity of tryptase Clara and the activation of virus infection. The 50% inhibitory concentrations of recombinant MPI and the C-terminal domain for tryptase Clara with Sendai virus envelope glycoprotein as substrate were 7.4 and 61.6 nM, respectively. These results indicate that MPI is a defensive compound against virus infection. Since there is evidence suggesting that concentrations of MPI in respiratory fluids are insufficient for prevention of virus infection, administration of MPI in the airway may be useful for treatment of these virus infections.
Url:
DOI: 10.1093/oxfordjournals.jbchem.a021588
Affiliations:
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Kido, Y. Yokogoshi, K. Sakai, M. Tashiro, Y. Kishino, A. Fukutomi, and N. Katunuma (1992) J. Biol. Chem. 267, 13573–13579]. We report here that human mucus protease inhibitor (MPI), a major inhibitor of granulocyte elastase in the lining fluids of the human respiratory tract, significantly inhibited proteolytic activation of the infectivity of influenza A and Sendai viruses by tryptase Clara in vitro and multi-cycles of mouse-adapted influenza A virus replication in rat lungs in vivo. Recombinant MPI and the C- but not the N-terminal domain of MPI inhibited both the proteolytic activity of tryptase Clara and the activation of virus infection. The 50% inhibitory concentrations of recombinant MPI and the C-terminal domain for tryptase Clara with Sendai virus envelope glycoprotein as substrate were 7.4 and 61.6 nM, respectively. These results indicate that MPI is a defensive compound against virus infection. Since there is evidence suggesting that concentrations of MPI in respiratory fluids are insufficient for prevention of virus infection, administration of MPI in the airway may be useful for treatment of these virus infections.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Ariga, Hiroyoshi" sort="Ariga, Hiroyoshi" uniqKey="Ariga H" first="Hiroyoshi" last="Ariga">Hiroyoshi Ariga</name><name sortKey="Beppu, Yoshihito" sort="Beppu, Yoshihito" uniqKey="Beppu Y" first="Yoshihito" last="Beppu">Yoshihito Beppu</name><name sortKey="Imamura, Yasuhiro" sort="Imamura, Yasuhiro" uniqKey="Imamura Y" first="Yasuhiro" last="Imamura">Yasuhiro Imamura</name><name sortKey="Kido, Hiroshi" sort="Kido, Hiroshi" uniqKey="Kido H" first="Hiroshi" last="Kido">Hiroshi Kido</name><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name><name sortKey="Towatari, Takae" sort="Towatari, Takae" uniqKey="Towatari T" first="Takae" last="Towatari">Takae Towatari</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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